Confocal Microscope System - Sources Sought Notice

Active

Contract Opportunity

The purpose of this sources sought notice is to conduct market research and identify potential sources of commercial products/services that satisfy the Government’s anticipated needs.

BACKGROUND

The National Institute of Standards and Technology (NIST), specifically the Biomedical Measurement Technologies group, is seeking an automated high-speed and high-resolution confocal scanning microscope. This microscope will be used for imaging biomolecules, such as proteins, DNA, and biological cells, on various substrates including quartz, silicon, and standard microscope slides.

The project aims to assess the surface coverage of fluorescently tagged biomolecules by measuring the total fluorescence in each field of view. In many cases, high spatial resolution (approaching 100 nm) is desired for these measurements to study localization on nanostructures on the substrate and will be accomplished by utilizing confocal microscopy. Since the focus is on molecules that are tagged, rather than cells that typically contain a high density of fluorophores, the signal-to-noise ratio (SNR) is critical for both confocal and widefield imaging. For confocal imaging this might be achievable by hardware and software capabilities that allow operating at higher effective pinhole sizes quantified by the light collection efficiency in Airy Units (AU), e.g., achieving the resolution of 0.3 AU confocal image with the SNR of a 1.2 AU pinhole. For widefield imaging the requirement for maximizing SNR might translate into using cameras with quantum efficiencies (QE) of 90 % or above. Additionally, it is essential to monitor the coverage of the receptors on the surface and their change over time, necessitating instrumentation with exceptional stability and the capability to track drift in focus and other instrument parameters.

The microscope must fulfill a wide range of requirements as outlined above, supporting various imaging modalities such as brightfield imaging, widefield fluorescence, phase contrast microscopy, total internal reflection fluorescence (TIRF) microscopy, and confocal scanning with high spatial resolution and fast scan rates. Additionally, the microscope should be part of a complete system that includes filter sets for multi-color imaging and light sources for bright field imaging, widefield fluorescence, and laser lines for confocal imaging from ≈400 nm to 640 nm that enable its use with common fluorophores.

The system should feature a high level of automation, allowing image capture in multiple modes, and be programmable for continuous monitoring over several days. The automation should also allow integration of other microscope features, such as focus tracking, to enhance long-term monitoring alongside image capture. Automation should also extend to microscope features such as motorized sample stages, nosepiece to allow changes in objectives and filters.

The group currently owns several fluorescence and confocal microscopes from Carl Zeiss Microscopy. Therefore, compatibility with these existing instruments—allowing for the exchange of objectives, condensers, filter cubes, and other consumables—will result in significant cost savings for the government, making it a crucial requirement for this project.

NIST is seeking information from sources that may be capable of providing a solution that will achieve the objectives described above, in addition to the following essential requirements:

• Single microscopy platform that allows bright imaging, widefield fluorescence imaging, confocal imaging and TIRF microscopy.


• Imaging with the highest SNR in all modes: high QE (>90 %) cameras for widefield imaging and high SNR and high-resolution imaging as described above.


• Flexibility to work with transparent and opaque samples in reflection mode.


• Exceptional stability and active features such as focus tracking for long term monitoring experiments.


• High level of system automation for both data collection and instrument control.

HOW TO RESPOND TO THIS NOTICE

In responding to this notice, please DO NOT PROVIDE PROPRIETARY INFORMATION. Please include only the following information, readable in either Microsoft Word 365, Microsoft Excel 365, or .pdf format, in the response: Submit the response by email to the Primary Point of Contact and, if specified, to the Secondary Point of Contact listed in this notice as soon as possible, and preferably before the closing date and time of this notice. Please note that to be considered for award under any official solicitation, the entity must be registered and “active” in SAM at the time of solicitation response.

• Provide the complete name of your company, address, name of contact for follow-up questions, their email, their phone number and, if your company has an active registration in https://sam.gov, your company’s Unique Entity ID (UEI).

• Identify the equipment that your company sells that can meet the objectives addressed in the BACKGROUND section of this notice. For each product recommended to meet the Government’s requirement, provide the following:
  
  
  
  • Manufacturer name
  
  
  • Model number
  
  
  • Technical specifications
  
  
  • If your company is not the manufacturer, provide information on your company’s status as an authorized reseller of the product(s)
  
  
  
  

• Describe performance capabilities of the product(s) your company recommends to meet the Government’s requirements. Additionally, if there are other features or functions that you believe would assist NIST in meeting its objectives described above, please discuss in your response.
  
  
  
  • Discuss whether the equipment that your company sells and which you describe in your response to this notice may be customized to specifications and indicate any limits to customization.
  
  
  
  

• Identify any aspects of the NIST market research notice, including instructions, and draft minimum specifications in the BACKGROUND section you cannot meet and state why. Please offer suggestions for how the market research notice and draft minimum specifications could be made more competitive.

• State whether the proposed equipment is manufactured in the United States and, if not, state the name of the country where the equipment is manufactured.

• Identify any plans/possibilities for changes in manufacturing location of the aforementioned equipment and provide relevant details, including timeline.

• For the NAICS code listed in this notice:

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  • Indicate whether your company is (a) a small business or (b) other than small business. See the Table of Small Business Size Standards and the associated .pdf download file for small business size standards and additional information.
  
  
  
  

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  • If you believe the NAICS code listed in this notice is not the best NAICS code for the type of product addressed in this notice, identify an alternative NAICS code that you believe would be more appropriate for the planned procurement.
  
  
  
  

• Describe services that are available with the purchase of the aforementioned equipment from your company such as installation, training, and equipment maintenance.

• Describe standard terms and conditions of sale offered by your company for the recommended equipment such as: delivery time after your company accepts the order; FOB shipping terms; manufacturer warranty (including details regarding nature and duration); if available, description(s) of available extended warranty; equipment setup and test; operator and service instruction manual(s); cleanup after installation; and if applicable, other offered services. Provide a copy of manufacturer standard terms and conditions that typically relate to the sale of the specified equipment, if available.

• State whether your company offers facility renovation services related to installation of the recommended equipment at its delivery destination, if required per the NIST-identified minimum specifications, and provide description of said services. Indicate if your company performs the facility renovation services or typically subcontracts the work to another company. Indicate if your company would be interested in inspecting the intended installation site during the market research phase.

• State published price, discount, or rebate arrangements for recommended equipment and/or provide link to access company’s published prices for equipment and services.

• If the recommended equipment and related services are available for purchase on any existing Federal Supply Schedule contract(s) or other contracts against which NIST may be able to place orders, identify the contract number(s) and other relevant information.

• Identify any customers in the public or private sectors to which you provided the recommended or similar equipment. Include customer(s) information: company name, phone number, point of contact, email address.

• Provide any other information that you believe would be valuable for NIST to know as part of its market research for this requirement.

• State if you require NIST to provide additional information to improve your understanding of the government’s requirement and/or would like to meet with NIST representatives to discuss the requirement and the capabilities of the identified equipment.

QUESTIONS REGARDING THIS NOTICE

Questions regarding this notice may be submitted via email to the Primary Point of Contact and the Secondary Point of Contact listed in this notice. Questions should be submitted so that they are received 5 calendar days prior to the response date. If the Contracting Officer determines that providing a written amendment to this notice to document question(s) received would benefit other potential respondents, the questions would be anonymized, and a written response to such question(s) would be provided via an amendment to this notice.

IMPORTANT NOTES

This notice is for market research purposes and should not be construed as a commitment by NIST to issue a solicitation or ultimately award a contract. There is no solicitation available at this time.

This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response.

NIST reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate.

Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization’s capability.

Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted.

After a review of the responses received, a synopsis and solicitation may be published on GSA’s eBuy or SAM.gov. However, responses to this notice will not be considered an adequate response to any such solicitation(s).

Thank you for taking the time to submit a response to this request!

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