scRNA sequencing service

The system utilizes a microfluidics based methods of sequencing.

. The workflow includes dead cell removal to ensure high data quality.

. The sample preparation workflow includes a protocol for maximizing cell viability.

. The sequencing system has .80% of bases with quality scores of Q30 or higher for 2x150 bp sequencing with an average that is preferably .90%.

. Workflow includes barcoding cells and droplet isolation using droplet sequencing methods.

. System utilizes semiautomated processing for surface marker enrichment and cell isolation.

. Sequencing workflow includes end repair, A-tailing, ligation and PCR.

. System provides flexible sequencing depth with a capture rate > 60%.

. System capable of profiling paired V(D)J transcripts with 5fgene expression and library preparation.

. Contractor provides a robust bioinformatics pipeline for visualization and cross referencing of sequencing data.