Solicitation of Interest - Total Internal Reflection Fluorescence (TIRF) Microscope

Solicitation of Interest
SUNY Upstate Medical University intends to contract for a TIRF Microscope to be used by our Biochemistry & Molecular Biology department.

Detailed Specifications
1. Microscopy Systems and Main Components
• Inverted Microscope Main Body
-Designed as an inverted microscope, suitable for examining samples from below (i.e., with the specimen positioned on the stage and the objective lenses positioned above)
• Controller for microscopy system or motorized equipment
• Motorized main branch for microscope systems
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2. Motion Control and Stage Systems
• Motorized Stage with XY Motion Capabilities
- Capable of providing motion in the XY plane, with a minimum stroke of 114mm in the X-axis and 75mm in the Y-axis.
-Must provide repeatable positioning accuracy of ±3 microns or better, with high precision in motion control
• Joystick Controller for Motorized Stage and Microscope Control
-Must provide precise XY control for motorized stage movement
• Motorized Condenser Turret for Microscopy
• Motorized Epi- Fluorescence Turret for Microscopy System
• Differential Interface Contrast (DIC) Analyzer Cube for Microscopy System
• Motorized Nosepiece with integral focus control for microscopy systems
- Must feature motorized rotation for smooth, precise switching between multiple objectives (e.g., 4x, 10x, 20x, 40x) in a microscope system
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3. Optical Components and Modules
• Epi-Fluorescence Illumination Module for Microscopy
• Fixed Main Branch for Microscopy System
• Ultra-High Performance TIRF (Total Internal Reflection Fluorescence Illumination System
-Must support multiple excitation wavelengths, including at least 405nm, 488nm, 561 nm, and 638nm, to cover a wide range of fluorophores for advanced fluorescence imaging
• Fiber Optic Light Source Adapter for Microscopy or Imaging Applications
• Eyepiece Tube Base Unit for Microscopy
• Photo Port Tube Base Unit for Microscopy Systems
• Exchangeable Mirror With 100% Reflectivity for Microscopy System)
• 10X Eyepiece for Microscopy
-Must offer a wide field of view, with a minimum of 22mm
• Ergonomic Binocular Viewing Tube for Microscopy
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4. Illumination Systems
• Transmitted LED Lamp House for Microscopy
• Diascopic Illumination Pillar for Microscopy
• TIRF (Total Internal Reflection Fluorescence) and Photostimulation Illuminator for Microscopy Systems
• Multi-wavelength laser combiner for fluorescence microscopy or Imaging Systems
-Must provide laser output at multiple wavelengths, ideally covering common excitation lines such as 405 nm, 488 nm, 561 nm, and 640 nm, to support a wide range of fluorophores
- Should provide laser power of at least 20 mW per wavelength, ensuring sufficient excitation intensity for fluorescence imaging and spectroscopy
-Include an Automatic Power Control (APC) feature to maintain stable laser power output over time, compensating for any power fluctuations or drift
• Light Engine for Fluorescence or Wide-Field Imaging Applications
- Must provide continuous light output across a broad spectrum, typically from 350 nm to 680 nm, covering key excitation wavelengths for a variety of fluorophores
• High-Performance Illuminator for Microscopy or Imaging Applications
- Cover a broad spectrum of wavelengths to support a variety of imaging applications, including excitation for fluorescence (typically 350 nm to 700 nm or a similar range depending on the application)
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5. Imaging and Camera Systems
• Camera System for the Back Aperture of a Microscope, designed for Fluorescence or High-Resolution Imaging
• Analog Triggered Device Hub for Integration with Microscopy or Laboratory Systems
• Back-Illuminated SCMOS (Scientific CMOS) Camera for Microscopy Imaging
- Must offer high-resolution imaging, with a minimum resolution of several megapixels (e.g., 5 MP, 6 MP, or higher), ensuring detailed capture of images for a wide range of microscopy applications
- Support high frame rates for capturing fast-moving phenomena, ideally at least 30 fps (frames per second) or higher at full resolution
• C-Mount/ISO Camera Adapter for Microscope System
- Provide a 1X optical magnification, ensuring that the camera captures the image at a 1:1 ratio, without additional zoom or reduction
• TIRF ( Total Internal Reflection Fluorescence Microscopy Driver and Software Integration Module
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6. Filters and Optical Accessories
• Emission Filter for Fluorescence Microscopy or Imaging Systems
-32mm, compatible with standard filter wheel or optical systems that support this size
- Designed for emission of fluorescence signals in the 595 nm range, typically used for fluorophores such as Texas Red, Alexa Fluor 594, or other dyes with similar emission characteristics
- Provide high transmission of light within the designated emission range (595 nm), with minimal light loss and optimal contrast for fluorescence imaging
• Single Bandpass (BP) Filter for Fluorescence Microscopy or Imaging Applications
-32mm, designed to fit standard filter wheels, optical cubes, or other filter holder systems that support this size
-Optimized for excitation or emission light in the 432 nm to 436 nm range (centered at 432 nm), suitable for use with fluorophores excited or emitting in this range, such as DAPI or other blue-emitting fluorophores
- Designed to pass light (approximately 36nm) within this narrow wavelength range while blocking out other unwanted wavelengths
- Must exhibit high transmission of light in the specified bandpass range (432 ±18 nm) to ensure optimal fluorescence detection and minimal light loss
• Quadruple Emission (EM) Filter for Multi-Channel Fluorescence Microscopy
-32mm, designed for use with standard filter wheels, optical filter cubes, or other systems that accommodate 32mm filters
- Must be designed to efficiently filter emission light for commonly used fluorophores in fluorescence microscopy, specifically:
DAPI (excitation ~358 nm, emission ~461 nm)
FITC (excitation ~495 nm, emission ~519 nm)
CY3 (excitation ~550 nm, emission ~570 nm)
CY5 (excitation ~649 nm, emission ~670 nm)
- Optimized for emission light in the wavelength ranges corresponding to DAPI, FITC, CY3, and CY5 fluorophores, providing high transmission for each of the emission bands and blocking other wavelengths
• Fluorescence Illumination System with Broad-Spectrum Capability for Microscopy Applications
-Capable of multiple wavelength outputs for fluorescence excitation, ideally covering commonly used fluorophores such as DAPI (typically around 350 nm), as well as other ranges (e.g., blue, green, and red) for multi-channel imaging
• Fluorescence Filter Set for Microscopy Applications
-Should be specifically designed for GFP (Green Fluorescent Protein) excitation and emission, as well as other commonly used fluorophores (e.g., DAPI, FITC), ensuring clear imaging in fluorescence microscopy
- Must include excitation and emission filters that are compatible with GFP (approximately 480 nm excitation, 510 nm emission), as well as other common fluorophore wavelength ranges (e.g., blue, green, and red)
• Fluorescence Filter Set for Red Fluorophores in Microscopy Applications
-Must be optimized for the excitation and emission of red fluorophores, such as those in the 600–650 nm range (e.g., Texas Red, Alexa Fluor 594, or similar), and should also support other common fluorophores used in multi-channel fluorescence imaging
-Include excitation and emission filters designed to work with red fluorophores, typically with excitation in the range of 540–620 nm and emission around 650–670 nm, ensuring high sensitivity and contrast for red fluorescence imaging
• Fluorescence Filter Set for Far-Red Fluorophores in Microscopy Applications
- Must be optimized for the excitation and emission of CY5 (or similar far-red fluorophores), typically with excitation in the range of 640–650 nm and emission around 665–680 nm
• Motorized or Manual Filter Wheel for Barrier Filters in Fluorescence Microscopy or Multi-Channel Imaging Applications
- Must be capable of supporting multiple filter positions (e.g., 4-5 positions) for holding different barrier filters, allowing for the selection of various wavelengths for multi-channel imaging
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7. Lenses and Objectives
• Plan Apochromat Objective Lens for Microscopy Systems
-Capable of providing a numerical aperture (NA) appropriate for the 20X magnification (typically around 0.75 or higher), ensuring adequate light-gathering capability for high-resolution imaging in transmitted light and fluorescence applications
- Capable of providing a suitable working distance for ease of use in a range of sample types and applications (working distance may typically be in the range of 0.5–1.0 mm, depending on the design)
• High-Performance Oil Immersion Objective Lens for TIRF (Total Internal Reflection Fluorescence) Microscopy
-60X, designed to provide high-resolution imaging for detailed, high-magnification observation in microscopy applications
- Numerical Aperture of 1.49, enabling high light-gathering power, essential for achieving excellent resolution and contrast in TIRF microscopy, and ensuring optimal fluorescence detection
- Extremely short working distance of 0.12 mm, optimized for high-magnification imaging where precise sample positioning is critical (suitable for TIRF imaging, which requires very close proximity to the sample surface)
- Designed to provide a flat, distortion-free field of view, essential for high-accuracy imaging at 60X magnification
• Differential Interference Contrast (DIC) Slider for Microscopy Systems
-20X, designed to provide medium-level magnification for high-quality imaging in DIC microscopy applications
• Differential Interference Contrast (DIC) Slider for High-Magnification Microscopy Systems
-60X, designed for high-magnification imaging, offering greater resolution for detailed observation of small structures in transparent or semi-transparent specimens
• Differential Interference Contrast (DIC) Module for Microscopy Systems
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8. Sample Holders and Stages
• Universal Adjustable Holder Compatible With Motorized Stages
• Well Plate Holder Compatible With Motorized Stages
- Must securely hold imaging dishes for integration with a motorized stage
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9. Connectivity and Cables
• Long Daisy Chain Cable for Connecting Microscopy or Laboratory Equipment
• External Trigger Cable for Synchronization of Imaging or Experimental Equipment
• Interlock Cable for Laboratory Equipment Safety and Synchronization
• Interlock Cable Designed for Use in Safety Systems and Automated Equipment Control, Ensuring Safe Operation by Providing Reliable Electrical Connections Between Interlock Devices and Systems
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10. Software and Licenses
• Advanced Imaging Software Suite for Microscopy Systems
• XY Galvo Scanning Module Designed for High-Precision Optical Control and Positioning in Scientific, Industrial, and Laboratory Applications
• Advanced 6D Imaging Software Suite
-Capable of supporting 6-dimensional (6D) imaging, combining multiple spatial dimensions (XYZ) with time (T) and up to three additional parameters, such as multi-channel fluorescence, multi-angle, or multi-focus imaging
-Supports simultaneous acquisition of XYZ spatial coordinates, time-lapse (T) imaging, and additional dimensions such as multi-channel fluorescence, multi-focus, and multi-angle imaging for comprehensive data collection
• Wavelength Switching System for Fluorescence Microscopy
• External Device Control and Synchronization Module for Microscopy Systems
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11. Safety and Accessories (included as a bundles item or ability to purchase as separate line items)
• Digital Gas Detector or Monitor
- Capable of measuring gas concentrations within a specified range, typically in ppm (parts per million), % volume, or other appropriate units, depending on the gases monitored
• Laser Safety Interlock Lid or Cover
• Interlock Junction Box Designed for Integrating and Managing Safety Interlocks in Laboratory, Industrial, or Research Systems
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12. Miscellaneous Components
• Comprehensive Installation Kit for Microscopy or Laboratory Equipment Setup
• igh-Performance Computing Workstation for Advanced Imaging Applications
• Ultra-Wide Curved Display for Professional Imaging, Data Visualization, and Multitasking Environments
-39.7” Ultra-Wide Curve Display
• Large-Format Package for Scientific, Industrial, or Laboratory Use, Providing Comprehensive Support or Setup for Specific Applications
-750x750mm package, offering a spacious and customizable setup area for working with larger or multiple items simultaneously
-30x30 grid configuration, allowing for a structured arrangement of components or specimens in rows and columns, ideal for high-throughput applications, systematic experimentation, or storage solutions
• Air Compressor Designed for use in Scientific, Laboratory, or Industrial Applications, Specifically for Vitrification (VIT) or Other Processes Requiring Controlled Air Supply
-110V, electrical system
-Capable of Providing consistent air flow and pressure, with specifications for CFM (Cubic Feet per Minute) or PSI (Pounds per Square Inch) that suit the needs of specific applications, such as the delivery of air for cooling, pressurizing, or drying in vit-related processes
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13. Optical and Light Components
• High-Performance Light Source or Laser Module for Scientific, Research, or Industrial Applications Requiring Specific Wavelength and Power Output
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14. Condenser and Lens System
• Long Working Distance (LWD) lens unit for microscope condenser turret system
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15. Extended Warranty
• Minimum 3-Year Warranty on Parts and Labor
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Minimum Qualifications of Eligible Organizations
• Vendor must be eligible to do business with New York State

How to Apply:
To indicate interest in and eligibility to provide this TIRF microscope, please submit a letter of interest describing the Responding Vendor's ability to meet the above requirements. The document must be submitted via e-mail to frigons@upstate.edu no later than 08:00am on 3/21/2025.