Bio-Rad QX600 Droplet Digital PCR System

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Contract Opportunity

Requirements

The laboratory requires a digital droplet PCR (ddPCR) platform capable of absolute nucleic acid quantification with high sensitivity and multiplex capability.

The quoted system provides up to six-color multiplex detection, processes up to 96 samples per run, and performs absolute nucleic acid quantification

Type of Order:

Firm Fixed Price Purchase Order

Special Order Requirements:

The instrument shall:

• Perform digital droplet PCR using partitioned droplet technology.

• Partition each 20 μL reaction into approximately 20,000 droplets.

• Support six-color fluorescence detection.

• Process 96-well plates.

• Provide absolute nucleic acid quantification without reliance on standard curves.

• Support applications including:

• mutation detection

• copy number variation

• gene expression

• viral titer analysis

• Include software capable of automated thresholding, Poisson analysis, multiplex analysis, and export of results.

• Include all required computer hardware and system software.

• Include a compatible thermal cycler capable of operating 96-well deepwell PCR plates.

These specifications are consistent with the manufacturer's published technical specifications.

The contractor shall deliver the following equipment to the National Institutes of Health (NIH):

• One (1) Bio-Rad QX600 Droplet Digital PCR System

• One (1) Bio-Rad PTC Harmony Deepwell Thermal Cycler

Training:

Training is not included under this purchase. The contractor is not required to provide user training as part of this award.

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