Sole Source Chapter PomePlex-26 NGS Assay (96 tests)

Sole Source Notification

Acquisition of 10 kits of the ChapterDx PomePlex 26

The Plant Germplasm Quarantine Program routinely screens for a battery of 26 viruses and viroids through individual PCR diagnostics for each of the 26 targets, which is very laborious and time consuming. The ChapterDx PomePlex 26 allows testing all 26 viruses and viroids all at once in a single tube reaction.

This technology will be used as part of the work plan approved for a project awarded by the Plant Protection Act PPA7721 Spending plan FY26 titled “Implementing a largescale assay for simultaneous diagnostic of all pome tree viruses/viroids in certification

programs”. The list of awarded projects can be found at Plant Protection Act Section 7721 Fiscal Year 2026 Spending Plan. Each of the 10 kits requested for purchasing provides enough reagents to conduct diagnostics on 96 individual samples across the 26

targeted plant pathogens.

ChapterDx is the only company that sells a 26 NGS assay ready for sequencing in a 96 sample format with as little as 2ul of samples when followed NGS-based techniques. Our laboratory conducts plant pathogen diagnostics of imported germplasm that are compatible with all our established workflows for accurate diagnostics of plant pathogens using the ChapterDx Pome Plex 26 NGS assay.

This notice closes September 07, 2026 12:00 PM CST

Todd K. Bierl

(Major Program Contracting Officer)

Emergency Acquisition

Marketing and Regulatory Program Business (MRPBS)

Acquisition and Asset Management Division (AAMD)

Acquisition Branch

Animal and Plant Health Inspection Service (APHIS)

Office#(612)336-3465

Cell# (612)500-1321/(763)286-8084