INTENT TO SOLE SOURCE - DNA EXTRACTION, AN AMPLICON LIBRARY PREPARATION AND SEQUENCING ENVIRONMENTAL DNA (eDNA) FILTERS

***THE NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION’S (NOAA) OCEANIC AND ATMOSPHERIC RESEARCH (OAR) PACIFIC MARINE ENVIRONMENTAL LABORATORY (PMEL) INTENDS TO NEGOTIATE, ON A SOLE SOURCE BASIS, UNDER AUTHORITY OF FAR 13.106-1(b)(1), WITH JONAH VENTURES LLC LOCATED AT BOULDER, CO TO PROVIDE THE A NON-PERSONAL SERVICE FOR DNA EXTRACTION, AN AMPLICON LIBRARY PREPARATION AND SEQUENCING ENVIRONMENTAL DNA (eDNA) FILTERS.

***THE SOLE SOURCE DETERMINATION IS BASED ON THE JONAH VENTURES LLC IS THE ONLY ONE SOURCE THAT IS REASONABLY AVAILABLE AS DETAILED BELOW: COMPATIBILITY TO EXISTING SYSTEMS OR EQUIPMENT – THE REQUIRED SERVICE DELIVERABLES MUST BE COMPATIBLE IN ALL ASPECTS (FORM, FIT, AND FUNCTION) WITH EXISTING SYSTEMS OR EQUIPMENT, AND THE SOURCE IS UNIQUELY QUALIFIED TO MEET THE REQUIREMENT. TO COMPARE TO EXISTING DATA, THE NEW DATA MUST BE GENERATED WITH THE SAME PLATFORM AND SEQUENCING CHEMISTRY, AS OUTLINED BELOW. THE FULL METHODOLOGICAL DETAILS OF THE SEQUENCE LIBRARY PREPARATION ARE UNIQUE TO JOHAH VENTURES LLC AND WOULD REQUIRE SIGNIFICANT EFFORT AND COOPERATION FROM THE COMPANY TO ACQUIRE THE PROTOCOLS AND FIND AN ALTERNATIVE CONTRACTOR WILLING AND ABLE TO IMPLEMENT THEM. FURTHERMORE, DATA DELIVERY MUST BE CONDUCING TO OUR BIOINFORMATIC WORKFLOW TO ENSURE RAPID AND ERROR-FREE PROCESSING, INCLUDING DEMULTIPLEXING OF INDIVIDUAL SAMPLES INTO FASTQ FORMATTED FILES. FOR THIS PROJECT, PMEL HAS DEVELOPED WORKFLOWS AND SCRIPTS TO SPECIFICALLY PROCESS AND ANALYZE THE DATA FORMATS PROVIDED BY THE VENDOR AND THUS SWITCHING VENDORS WOULD LEAD TO INEFFICIENCIES AND DELAYS IN DELIVERING SCIENCE PRODUCTS. LASTLY, THE VENDOR MUST HAVE A PROVEN TRACK RECORD OF SUCCESS SEQUENCING eDNA FROM FILTERED MARINE WATERS AS WELL AS A TRACK RECORD OF WORKING WITH AMPLICON SEQUENCING AND MICROBIOME SEQUENCING.

PERIOD OF PERFORMANCE: APRIL 01, 2025 TO MARCH 31, 2026

***THIS NOTICE OF INTENT IS NOT A REQUEST FOR COMPETITIVE PROPOSALS AND NO SOLICITATION PACKAGE IS AVAILABLE. HOWEVER, FIRMS THAT BELIEVE THEY CAN FULLY MEET THE GOVERNMENT'S REQUIREMENTS MAY SUBMIT SUBSTANTIATING DOCUMENTATION IN WRITING TO THE IDENTIFIED POINT OF CONTACT WITHIN 15 DAYS AFTER PUBLICATION OF THIS SYNOPSIS. SUCH DOCUMENTATION WILL BE EVALUATED SOLELY FOR THE PURPOSE OF DETERMINING WHETHER OR NOT TO CONDUCT THIS PROCUREMENT ON A COMPETITIVE BASIS. A DETERMINATION BY THE GOVERNMENT NOT TO CONDUCT A COMPETITIVE PROCUREMENT, BASED UPON RESPONSES TO THIS NOTICE, IS SOLELY WITHIN THE DISCRETION OF THE GOVERNMENT. ORAL COMMUNICATIONS ARE NOT ACCEPTABLE IN RESPONSE TO THIS NOTICE.