ACAN 2025-0145 BD FACSDiscoverS8

Location:	Alberta
Posted:	Sep 3, 2025
Due:
Agency:	Government of Alberta
Type of Government:	State & Local
Category:	A - Research and development
Solicitation No:	AB-2025-05779
Publication URL:	To access bid details, please log in.

Advanced Contract Award Notice / Notice of Proposed Procurement

University of Alberta

The University of Alberta is providing Advanced Contract Award notice to acquire a BD FACSDiscoverS8 Cell Sorter Imaging Instrument from Becton Dickinson Canada. During investigation of equipment phase of the granting process the Faculty of Medicine has invited all interested manufactures or their representative to demonstrate the functionality of their instruments. This has resulted in the discovery of only one instrument with unique capabilities that will benefit the various researchers that will utilize the instrument. This instrument will be installed in the core facilities that is not lab or researcher specific. This is a shared resource and will be used for diverse research goals.
the BD FACSDiscoverS8 enables not only a direct visual of cells to be sorted in real time and provides a record (image) of each cell sorted, but it also allows the operator to sort cells based on imaging parameters. No other instrument does this. This imaging sorting feature is required because it offers a level of quality control that is not possible on any other cell sorter. In addition to being able to visually inspect cells through light loss (similar to brightfield) images to confirm they are indeed cells - not debris - and that they look healthy, up to three fluorescent markers and a variety of morphological (shape) characteristics can be used to sort cells, and can be combined with up to 50 spectral parameters.
Technical aspects of the BD FACSDiscover that are unique to this instrument include:
•        Real-time imaging (6 imaging detectors on the blue laser, 12 imaging parameters) for image-enabled cell sorting. BD CellView™Image Technology produces images without a camera by using orthogonal frequency domain multiplexing with the flow cytometer's electronic and optical components; this allows images to be generated quickly enough for sorting. Up to three fluorescence channels off the blue laser can be employed. Imaging parameters include correlation, delta center of mass, diffusivity, eccentricity, maximum intensity, radial moment, center of mass (x), center of mass (y), forward scatter, side scatter, light loss, and total intensity.
•        Full spectrum optics (spectral cytometry) with next generation quality control and software algorithms that adapt to instrument performance and sample conditions in real time to properly unmix spectral signatures - this results in clean data with which to make sort decisions
•        Avalanche photodiode (APD) fluorescence detector arrays combined with optimized filter bandwidths that cover the full spectrum with no gaps (5 lasers and 78 detectors with full coverage ranging 365 - 860 nm)
•        Reliable plate sorting into both 96-well and 384-well plates; deposition of single cells into each well of a 96-well plate can be achieved in under 80 seconds (with 100 nm nozzle at 20 psi)
•        Small particle detection capability: image of 0.2 µm polystyrene bead resolved from noise with at least one side scatter parameter

APC Reference # AB-2025-05779

Category Goods

Posting Date • Time Sep 3, 2025 • 9:13 am (MT)

Region(s) of Delivery Alberta

Set Bookmark

Share Link